okadaic acid solubility

0.1mg. EN-141 EN-140 EN-139 EN-138 EN-137 EN-136 EN-135 EN-134 EN-133 EN-132. Useful for the Product Name Okadaic Acid Product Number 5934 Identified Uses For Research Use Only (RUO). Lipoic acid (LA), also known as α-lipoic acid, alpha-lipoic acid (ALA) and thioctic acid, is an organosulfur compound derived from caprylic acid (octanoic acid). Size: 100 µg. Okadaic acid is a diarrhetic shellfish toxin initially produced by dinoflagellates and sea sponges; it exhibits neurotoxic and anticancer activities. Inhibits protein phosphatases 1 and 2A. General Description This protocol specifies a method for the quantitative determinat ion of Okadaic Acid (OA) and other carboxylic toxins of the OA group including DTX1, DTX2 and DTX3 by a colorimetric phosphatase inhibition assay. N/A. Okadaic acid at 0.05 nmol significantly increased pCREB and pElk-1. Okadaic acid (10 nM) inhibited PP-2A activity and okadaic acid (100 nM) inhibited both PP-2A and PP-1 activity. Okadaic acid is a widely distributed marine toxin produced by several phytoplanktonic species and responsible for diarrheic shellfish poisoning in humans. OA group toxins were detected in mollusk by UPLC-MS/MS with the highest level of 11.3 ng/g and detection frequency of 11.8%. Okadaic acid is a lipophilic marine algal toxin commonly responsible for diarrhetic shellfish poisoning (DSP). Solubility ( literature ): Reportedly, Okadaic acid is soluble in acetone, methanol, ethanol, chloroform or ethyl acetate. 1-4. OKADAIC ACID Specification. Okadaic acid (OA) and its derivatives, which are produced by dinoflagellates of the genera Prorocentrum and Dinophysis, are responsible for diarrhetic shellfish poisoning in humans.In laboratory animals, these toxins cause epithelial damage and fluid accumulation in the gastrointestinal tract, and at high doses, they cause death. No. Okadaic acid, Potassium salt . Displays > 100,000,000-fold selectivity over PP2B and PP2C. At the molecular level, Okadaic acid is a pot ent and specific inhibitor of various types of serine/threonine protein phosphatases. Okadaic Acid (OA) is a fat soluble toxin that can accumulate in bivalves and, if ingested, may cause diarrhetic shellfish poisoning (DSP), an affliction that is characterized by incapacitating diarrhea, nausea, vomiting, abdominal cramps and chills. In higher concentrations Okadaic acid induced apoptosis (IC 50 = 0.5 µM). Okadaic acid Instruction Manual. Non-phorbol type tumor promoter. Okadaic acid (10 nM) inhibited PP-2A activity and okadaic acid (100 nM) inhibited both PP-2A and PP-1 activity. Finally, okadaic acid is soluble in different solvents, so the influence of this parameter on the results of these calculations can also be addressed. ... the cell lysate was neutralized and centrifuged at 10,000 rpm for 10 min to separate formic acid soluble and insoluble fraction (see Experimental procedures). okadaic acid sodium salt,okadaic acid sodium salt from prorocentrum concavum,okadaic acid sodium salt … 520025 1. Manufacturer/Supplier Cell Signaling Technology, Inc. 3 Trask Lane Danvers, MA … Okadaic acid inhibits protein phosphatases 1 and 2A (PP1, PP2A). Collected OA was measured by high-performance liquid chromatography with fluorimetric detection after derivatization with 1 … Okadaic acid, Sodium salt ; BV-1764 . Tumor promoter. It is a reversible inhibitor. The solubility of okadaic acid (OA) in supercritical CO 2 was measured using a flow-type apparatus with sequential sampling during dynamic nonrecirculating experiments at saturation conditions. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 10-1000 nM for 15-60 min. 520025 2. Cells were exposed to 400 nM okadaic acid for 30, 60, 75 and 90 min before they were either fixed ( A , C , E , G ) or the toxin was removed ( B , D , F , H ). MDL Number. Okadaic acid is a potent inhibitor of protein phosphatase 1 (IC 50 = 3 nM) and protein phosphatase 2A (IC 50 = 0.2-1 nM). Solubility White crystalline solid. Lipophilic okadaic acid is a potent inhibitor of protein phosphorylase phosphatase-1 and -2A in the cytosol of the mammalian cells that dephosphorylates serine and threonine. Okadaic acid, C44H68O13, is a toxin produced by several species of dinoflagellates, and is known to accumulate in both marine sponges and shellfish. Solubility: Soluble in DMSO, ethanol, methanol, acetone, ethyl acetate or distilled water. J Neurosci Methods 319:60-68 (2019). Other Product Data: Salt form of okadaic acid (Prod. Catalog # 27047. 25 µg 100 µg ; Soluble in DMSO or Ethanol. 1543-025) with somewhat greater stability than the free acid when stored in organic solvents. Description. and Prorocentrum spp., and the lead compound of diarrhetic shellfish poisoning (DSP) toxins which are highly lipophilic (EFSA 2008).Therefore, OA and its analogs occur in the fatty tissue and the hepatopancreas of organisms, whose diet is based on the extraction of … Okadaic acid also induced the expression of p53 and bax, which are necessary for the apoptotic programs. Okadaic acid (OA) and microcystins (MCs), which are the most common cyanobacterial toxins found in the water systems, from fresh to brackish waters, are structurally unrelated compounds produced by phylogenetically unrelated species which are known to inhibit … Okadaic acid, is used as an activator of PKC and inhibitor of PP1 and PP2A phosphatases. Okadaic acid (OA)’ is a polyether of 38-carbon fatty acid, originally isolated from the sea sponge Halichodrza okadai (1). Okadaic Acid (PP2A), Microtiter Plate Test for the Detection of Okadaic Acid-toxins group Product No. Not certified 100 µg 1 mg Okadaic acid LKT-O4101 Purity ≥98 % 25 µg Soluble in DMSO, ethanol, or methanol - Not certified 50 µg and Prorocentrum spp., and the lead compound of diarrhetic shellfish poisoning (DSP) toxins which are highly lipophilic (EFSA 2008).Therefore, OA and its analogs occur in the fatty tissue and the hepatopancreas of organisms, whose diet is based on the extraction of … BYHIFOCTDVNQQT-GHIYGBLASA-M. Synonym. ; Synonyms: Okadaic Acid, Sodium Salt - Calbiochem; find Sigma-Aldrich-459620 MSDS, related peer-reviewed papers, technical documents, similar products & … In N/N1003A cells, okadaic acid (10 nM) decreased total phosphatase activity by 20% and mainly inhibited PP-2A activity, while okadaic acid (100 nM) reduced 81% total phosphatase activity and inhibited PP-1 and PP-2A activity. Chem a. of, derived from, or containing acid b. being or having the properties of an acid 4. Figure Lengend Snippet: Okadaic acid-induced cell rounding is not directly coupled to cell death. It is a metabolite of Prorocentrum lima. Okadaic acid is a polyether compound isolated from the black sponge Halichondria okadai. It has various effects on biochemical parameters in intact cells: initiation of smooth muscle contraction, stimulation of glucose output and gluconeogenesis in … Okadaic acid, C 44 H 68 O 13, is a toxin produced by several species of dinoflagellates, and is known to accumulate in both marine sponges and shellfish. One of the primary causes of diarrhetic shellfish poisoning, okadaic acid is a potent inhibitor of specific protein phosphatases and is known to have a variety of negative effects on cells. Not certified . It was named from the marine sponge Halichondria okadai, from which okadaic acid was isolated for the first time. $165. White solid or clear colorless film. In animal models of Alzheimer’s disease, okadaic acid increases phosphorylation of … Solubility. Not intended for therapeutic or diagnostic procedures. AG-CN2-0056) with slightly greater stability than the free acid after it is put into stock solution (in organic solvents). Okadaic acid is a potent and selective inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Use freshly. The hydrophobic backbone of okadaic acid enables it to enter cells where it stimulates intracellular protein phosphorylation. Okadaic acid inhibits protein phosphatases 1 and 2A (PP1, PP2A). The process was dose dependent. Puromycin dihydrochloride allows selection for cells expressing the resistance gene puromycin N-acetyl-transferase (PAC) and is useful in CRISPR/Cas9 mammalian gene editing by selecting for successful Cas9-induced knock-in with puromycin resistance gene.

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